Version 5.4
Interaction: TIA1 :: TIAL1 XML
Summary
InnateDB Group ID 219740
Short name TIA1 :: TIAL1
Full name TIA1 interacts with TIAL1
Interaction type transcriptional regulation
Participants
Participant 1
Molecule: IDBG-55200
Molecule type: protein
Species: Homo sapiens
HUGO: TIA1
Full name: TIA1 cytotoxic granule-associated RNA binding protein
Direction: input
Biological role: unspecified role
Participant 2
Molecule: IDBG-91185
Molecule type: rna
Species: Homo sapiens
HUGO: TIAL1
Full name: TIA1 cytotoxic granule-associated RNA binding protein-like 1
Direction: input
Biological role: unspecified role
Evidence Reference 1
InnateDB ID IDB-187561.1
Last Modified 2010-03-28
Review status reviewed [Report errors or provide feedback]
Source database INNATEDB
Source database ID IDB-187561
PMID 17620417
Interaction detection method coimmunoprecipitation
Host system invitro
Host organism Homo sapiens
Cell status cell line
Cell line HeLa
Cell type epithelial cell
Tissue type cervical cancer cell line
Comment The TIAL1 mRNA was found to be enriched in the TIA1 immunoprecipitation relative to control immunoprecipitation (twofold or higher). To determine the functional significance of TIA1 binding to TIAL1 mRNA, the effect of down-regulation of TIA1 on TIAL1 expression was assessed by siRNA knockdown of TIA1. Knockdown of TIA1 expression by its specific siRNA resulted in a significant increase in TIAL1 protein levels.
Participants
Participant Identification Method Experimental Role Participant Accession
1) TIA1 western blot bait
2) TIAL1 primer specific pcr prey NM_001033925
Evidence Reference 2
InnateDB ID IDB-187562.1
Last Modified 2010-03-28
Review status reviewed [Report errors or provide feedback]
Source database INNATEDB
Source database ID IDB-187562
PMID 17620417
Interaction detection method affinity chromatography technology
Host system invitro
Host organism Homo sapiens
Cell status cell line
Cell line HeLa
Cell type epithelial cell
Tissue type cervical cancer cell line
Comment Biotinylated transcripts corresponding to either the 3â?² untranslated region (UTR) or the coding region (CR) of TIAL1 was incubated with whole-cell lysates. TIA1 associated with the biotinylated TIAL1 transcript and displayed much stronger binding to the 3â?² UTR than the CR fragment. To determine the functional significance of TIA1 binding to TIAL1 mRNA, the effect of down-regulation of TIA1 on TIAL1 expression was assessed by siRNA knockdown of TIA1. Knockdown of TIA1 expression by its specific siRNA resulted in a significant increase in TIAL1 protein levels.
Participants
Participant Identification Method Experimental Role Participant Accession
1) TIA1 western blot prey
2) TIAL1 predetermined participant bait NM_001033925

If you use InnateDB for your research, please cite the following publication:

Breuer et al., InnateDB: systems biology of innate immunity and beyond - recent updates and continuing curation. Nucl. Acids Res. (2013) 41 (D1) 

InnateDB is being developed jointly by the Brinkman Laboratory (Simon Fraser University, British Columbia, Canada), the Hancock Laboratory (University of British Columbia, Vancouver, British Columbia) and the Lynn EMBL Australia Group (South Australian Health & Medical Research Institute and Flinders University, Adelaide, Australia).

Funding is currently provided by Allergen and EMBL Australia. Previous funding has been provided by Genome Canada, the Foundation for the National Institutes of Health through the Grand Challenges in Global Health initiative and by Teagasc. InnateDB curated interactions are licensed under the Design Science License. All other data is licensed under the terms of the originating database. Contact: innatedb-mail@sfu.ca